Affinity chromatography is a separation process used to purify molecules or a group of molecules that are in a biochemical mixture. It employs two phases; a stationary phase and a mobile phase ...

The protocol describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorption immobilized-metal affinity chromatography. The starting material for the ...

Protocol Published: 08 February 2007 Purification of recombinant membrane proteins tagged with calmodulin-binding domains by affinity chromatography on calmodulin-agarose: example of nicotinamide ...